Abstract Clinical updates Coaxial TV Cable suggest conserving metastatic sentinel lymph nodes (SLNs) of breast cancer (BC) patients during surgery; however, the immunoadjuvant potential of this strategy is unknown.Here we leverage an immune-fueling flex-patch to animate metastatic SLNs with personalized antitumor immunity.The flex-patch is implanted on the postoperative wound and spatiotemporally releases immunotherapeutic anti-PD-1 antibodies (aPD-1) and adjuvants (magnesium iron-layered double hydroxide, LDH) into the SLN.Genes associated with citric acid cycle and Cards oxidative phosphorylation are enriched in activated CD8+ T cells (CTLs) from metastatic SLNs.
Delivered aPD-1 and LDH confer CTLs with upregulated glycolytic activity, promoting CTL activation and cytotoxic killing via metal cation-mediated shaping.Ultimately, CTLs in patch-driven metastatic SLNs could long-termly maintain tumor antigen-specific memory, protecting against high-incidence BC recurrence in female mice.This study indicates a clinical value of metastatic SLN in immunoadjuvant therapy.